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冠突伪尾柱虫小核对胞口结构稳定性的影响

摘要显微外科发觉冠状假性撒尿柱 克里斯蒂塔)无小核系中,学派细胞因口围带掉换部小膜呈现区分依序排列的错误而致使不合常规的现象或事件;在细胞长安息期的群体中,口围带不合常规的现象或事件率高达50%前述事项;对无小核系600多个刚完成或结束二分配的前、后费力查出和1 1000多种虫生理最后阶段蛋白质的银染,除4只二歧化费力查出和3只二歧化费力查出装腔作势地说周带非常外,压倒的多数表示方式整队发作后的细胞都具有规则的胞口建筑物.在另一方面,专一性培育试验更远地宣布,装腔作势地说周不合常规的现象或事件发作在非整队发作阶段。,耽搁小核的细胞,同时也耽搁了胞口建筑物的稳定性.To explore the generality of the morphogenetic function of the micronucleus among ciliates, we employed five stocks of hypotrichous ciliate Pseudourostyla 克里斯蒂塔 : 2, 4, 6, 28 and 46, and five a小核 cell lines:2aml, 4aml, 6aml, 28aml and 46aml derived from stocks 2, 4, 6, 28 and 46 respectively, which were generated by amputation of the cell when the micronuclei became aligned on the left side of the amalgamated macronuclei during cell 机关。 小核 cell lines 2ml, 4ml, 28ml and 46ml derived after amputation but retaining the micronuclei were also studied and served as 把持。 For observation of the cortex and nuclei, protargol staining was used 从头到尾。 The evident character of the a小核 cell lines, as compared with the normal and the 小核 cell lines, was that a substantial proportion of cells exhibited an abnormal adoral zone of membranelles (阿兹姆) When the culture medium began to age after 7~10 days, the culture soon entered stationary phase of 增长。 During the period abnormal AZMs were observed in over 50% of a小核 cells in various cell lines, among which the percentage of 6aml cells with defective AZM came up to 98%. In fact, the percentage of cells with abnormal AZM in a culture population was changeable and varied from low to high from exponential growth phase to stationary 阶段。 These abnormal patterns involved various degrees of reduction of membranellar width in the anterior and middle part of the AZM lapel (阿兹姆 L), and in severe cases disruption of AZM continuity due to the loss of some membranelles, and also indentation of the cytoplasm in this 区域。 Morphogenesis of the a小核s in binary fission and in physiological reorganization was 检查。 Restructuring of the pre existing oral apparatus, both during binary fission in the putative proter and during physiological reorganization, resulted in the formation of a normal 阿兹姆。 After restoration of normal AZM in binary fission or physiological reorganization, abnormal AZM pat

作者金立培钟金华JIN Li Pei JIN Hua Zhong (神学院学生 of Life Sciences, Zhongshan University, Guangzhou 510275, 奇纳)
出处《坏蛋日记》SCIECASCSCD北京大学的胸部
2002年第2期 258-263,共六页
Acta 坏蛋学 Sinica

基金国家自然科学基金

关键词小判定胞口建筑物稳定性腹部纤毛虫的冠突伪尾柱虫无性分配口部发作Hypotrichous ciliate ( Pseudourostyla 克里斯蒂塔 ), Binary fission, Physiological reorganization, Stomatogenesis, Micronucleus


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